Molecular Genetics Lab, General Clinical Research Center at the University of Minnesota

The GCRC Molecular Genetics Core Laboratory (GCRC-MGCL) at the University of Minnesota is dedicated to providing instrumentation and techniques for clinical investigation of genetic disease and genetic susceptibility to disease. These techniques include:

Members of the GCRC-MGCL will help in the design of your project before submission to the GCRC. If you have any questions, or wish to know how the GCRC-MGCL can help you in your research needs, please contact William S. Oetting at bill@lenti.med.umn.edu.

William S. Oetting, Ph.D.
Department of Medicine
Box 485 Mayo
420 Delaware St. S.E.
University of Minnesota
Phone 612-624-1139

The GCRC-MGCL has a complete genetic linkage facility for gene mapping. Facilities include DNA extraction, and/or production of lymphoblastoid cell lines, STRP marker production and analysis and linkage analysis. For gene mapping, we can produce either a general genome screen (8A set of markers, 25 cM average distance) or a more complete genome screen (9 set of markers, 10 cM average distance). We have created a custom database that allows for the tracking of all samples, as well as allowing for inputting of family data and affection status for the eventual production of linkage files used in linkage analysis. STRP production is done using the LI-COR automated fluorescent DNA sequencer. For a more complete description of our linkage facility, go to this link

The DNA analysis facility

The need to analyze DNA sequences and detect variation in selected populations is important to understanding poteintial genetic contributions to the epidemiology of disease. We can provide DNA sequencing and DNA variant analysis. DNA sequence analysis can also help in struture:function determinations. Techniques we have to offer include fluorescent DNA sequencing, ASO analysis and other PCR based techniques.